Kronos HT WSL-1565

High throughput real-time luciferase assay system

  • High throughput live cell realtime reporter assay
  • Measuring with temperature and CO2 controlled incubation chamber
  • 24 well plate x 2 or 96 well plate x 2 (option)
  • Data display with real-time
  • Detectors scan each well, so culture plates don’t move.
  • Lower noise by PMT cooling
  • Dual-color luciferase assay by optical filter auto-changing

 

Rett Syndrome like phenotypes in the R255X Mecp2 mutant mouse are rescued by MECP2 transgene

mbd_white

Rett syndrome is a severe neurodevelopmental disorder that is usually caused by mutations in Methyl-CpG-binding Protein 2 (MECP2). Four of the eight common disease causing mutations in MECP2 are nonsense mutations and are responsible for over 35% of all cases of RTT. A strategy to overcome disease-causing nonsense mutations is treatment with nonsense mutation suppressing drugs that allow expression of full length proteins from mutated genes with premature in-frame stop codons.

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Common polymorphisms in WNT10A affect tooth morphology as well as hair shape

WNT10ANews

Hair and teeth are appendages of ectodermal origin, and there are common molecular backgrounds involved in their formation. To date, it has been revealed that a nonsynonymous polymorphism in EDAR has effects on the morphological variation in both hair and teeth. Previous association studies have confirmed that single nucleotide polymorphisms (SNPs) in/near THADA, FRAS1, WNT10A, NAF1 and FGFR2 are associated with hair morphology.

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Epistasis Constrains Mutational Pathways of Hemoglobin Adaptation in High-Altitude Pikas

hemoglobin count

A fundamental question in evolutionary genetics concerns the roles of mutational pleiotropy and epistasis in shaping trajectories of protein evolution. This question can be addressed most directly by using site-directed mutagenesis to explore the mutational landscape of protein function in experimentally defined regions of sequence space.

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